Differentiation of Bacillus anthracis , Bacillus cereus , Bacillus 1 thuringiensis based on csaB gene reflect their hosts ’ source
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چکیده
4 Jinshui Zheng, Donghai Peng, Xiaoling Song, Lifang Ruan, Jacques Mahillon and 5 Ming Sun 6 7 8 State Key Laboratory of Agricultural Microbiology, College of Life Science and 9 Technology, Huazhong Agricultural University, Wuhan 430070, China, and 10 Laboratoty of Food and Environmental Microbiology, Université catholique de 11 Louvain, B-11348 Louvain-la-Neuve, Belgium. 12 13 14 *Corresponding author: Sun Ming, E-mail: [email protected]; Tel: 15 86-27-87283455; Fax: 86-27-87280670. 16 17 18 19 20 21 Running title: csaBbased discrimination among Bc group strains 22 23 24 25 26 27 28 29 30 Copyright © 2013, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.00591-13 AEM Accepts, published online ahead of print on 5 April 2013
منابع مشابه
Cloning and nucleotide sequence analysis of gyrB of Bacillus cereus, B. thuringiensis, B. mycoides, and B. anthracis and their application to the detection of B. cereus in rice.
As 16S rRNA sequence analysis has proven inadequate for the differentiation of Bacillus cereus from closely related species, we employed the gyrase B gene (gyrB) as a molecular diagnostic marker. The gyrB genes of B. cereus JCM 2152(T), Bacillus thuringiensis IAM 12077(T), Bacillus mycoides ATCC 6462(T), and Bacillus anthracis Pasteur #2H were cloned and sequenced. Oligonucleotide PCR primer se...
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